*Target (protein/gene name): Brugia malayi, glutathione reductase, putative *NCBI Gene # or RefSeq#: 240339 *Protein ID (NP or XP #) or Wolbachia#: Bm1_04825 *Organism (including strain): Brugia malayi *Background/Disease Information (sort of like the Intro to your Mini Research Write up):
Brugia malayi is a nematode that is known for causing lymphatic filariasis (elephantiasis). It is common in South and South East Asia, especially abundant in the tropical regions. B. mayali are spread through infections, unsanitation, and even mosquito bites that transfer the parasites. Link to TDR Targets page (if present): http://www.tdrtargets.org/targets/view?gene_id=240339 Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) http://www.ncbi.nlm.nih.gov/pubmed/7032915 Essentiality of this protein:
Bm1_04825 has essentiality data
Gene/Ortholog: mtu2902 (OG4_10249); Phenotype: essential; Source study: nmpdr
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: blattner
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: essential; Source study: gerdes
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: keio
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: shigen
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: no significant loss or gain of fitness in bloodstream forms (3 days); Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: significant loss of fitness in bloodstream forms (6 days); Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: no significant loss or gain of fitness in procyclic forms; Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford *EC#: 1.8.1.7 Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.8.1.7
Figure 1: Reaction mechanism of glutathione reductase Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): No enzyme assay available Structure Available (PDB or Homology model) -- PDB # 3GRS Expression Information (has it been expressed in bacterial cells): Yes Purification Method: Affinity chromatography *length of your protein in Amino Acids 480 amino acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website 50271 Da
*NCBI Gene # or RefSeq#: 240339
*Protein ID (NP or XP #) or Wolbachia#: Bm1_04825
*Organism (including strain): Brugia malayi
*Background/Disease Information (sort of like the Intro to your Mini Research Write up):
Brugia malayi is a nematode that is known for causing lymphatic filariasis (elephantiasis). It is common in South and South East Asia, especially abundant in the tropical regions. B. mayali are spread through infections, unsanitation, and even mosquito bites that transfer the parasites.
Link to TDR Targets page (if present):
http://www.tdrtargets.org/targets/view?gene_id=240339
Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)
http://www.ncbi.nlm.nih.gov/pubmed/7032915
Essentiality of this protein:
Bm1_04825 has essentiality data
Gene/Ortholog: mtu2902 (OG4_10249); Phenotype: essential; Source study: nmpdr
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: blattner
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: essential; Source study: gerdes
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: keio
Gene/Ortholog: eco3379 (OG4_10249); Phenotype: non-essential; Source study: shigen
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: no significant loss or gain of fitness in bloodstream forms (3 days); Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: significant loss of fitness in bloodstream forms (6 days); Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: no significant loss or gain of fitness in procyclic forms; Source study: alsford
Gene/Ortholog: Tb927.10.10390 (OG4_10249); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
*EC#: 1.8.1.7
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.8.1.7
Figure 1: Reaction mechanism of glutathione reductase
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
No enzyme assay available
Structure Available (PDB or Homology model)
-- PDB # 3GRS
Expression Information (has it been expressed in bacterial cells): Yes
Purification Method: Affinity chromatography
*length of your protein in Amino Acids 480 amino acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website 50271 Da